KMID : 1134820140430101565
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Journal of the Korean Society of Food Science and Nutrition 2014 Volume.43 No. 10 p.1565 ~ p.1570
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Optimization of Extraction Conditions of Pleurotus cornucopiae by Response Surface Methodology
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Lee Hye-Jin
DO Jung-Ryong Chung Min-Yu Kim Hyun-Ku
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Abstract
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Response surface methodology (RSM) was used to optimize extraction conditions of Pleurotus cornocopiae. Coefficients of determination (R2) for dependent variables ranged from 0.86 at 0.91. Maximum extraction yield was 40.81% under the following conditions: microwave power of 60.08 watts, ethanolic concentration of 12.33%, and extraction time of 6.86 min. Maximum extraction electron donating ability was 35.72% at 44.13 watts, 19.30%, and 4.21 min. Maximum extraction superoxide dismutase (SOD)-like activity was 34.87% at 114.01 watts, 65.88%, and 1.56 min. Maximum extraction total polyphenol content was 31.77 mg/g at 50.52 watts, 23.00% and 2.90 min. Based on the superimposition of four dimensional RSM with respect to extraction yield, electron donating ability, SOD-like activity, and total polyphenol content obtained under various extraction conditions, the optimum ranges of extraction conditions were as follows: microwave power of 71.48¢¦92.84 watts, ethanol concentration of 55.01¢¦71.66%, and extraction time of 3¢¦9 min.
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KEYWORD
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Pleurotus cornucopiae, response surface methodology (RSM), electron donating ability, total polyphenol content
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